Genetic Variation Analysis among different sheep Breeds of Balochistan by Utilizing Cytochrome b Gene of Mitochondrial DNA.

Abstract

This study was conducted to analyze the utility of mitochondrial cytochrome b gene sequences for phylogenetic estimates of relationships between Sheep breeds of balochistan. 100 animals (4 sheep breeds) were selected from various areas of Balochistan. Blood samples were collected and processed for DNA extraction. Specific primers were designed for the amplification of mitochondrial cytochrome b gene. Direct Sequencing of gene was done to identify the genetic variation and phylogenetic relationship among sheep breeds. The genetic variations identified in three sheep breeds i.e. 243bp T/C whereas TAT/TAC which coded same amino acid Tyrosine on 81^st codon, 309bp T/C whereas TAT/TAC which coded same amino acid Tyrosine on 103^rd codon and 366bp G/A whereas GCG/GCA which coded Alanine on 122^nd codon in Harnai, Rakhshani and Balochi respectively. Phylogenetic tree was constructed on line by using MABL (Methods Algorithems Bio-informatique Lirmm) on line. Genetic distance was found among the breeds was 0.3.